Molecular Bio Reagent One Step Direct Pcr Kits Animal Tissue PCR Kit With UNG

Molecular Bio Reagent One Step Direct Pcr Kits Animal Tissue PCR Kit with UNG

Product Descprition

Animal Tissue Direct PCR Kit(65℃ lysis) -UNG uses a unique lysis buffer system to quickly release genomic DNA from animal tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as template for PCR reaction.

2×PCR Easy^TM Mix (UNG) uses dUTP instead of dTTP on the basis of 2×PCR Easy^TM Mix, and adds UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of PCR products contamination during large-scale genetic testing.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

Product components:

Kit application:

- Application: a variety of animal tissues.

- DNA released from sample lysis: only used as a PCR template.

- The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

Kit Limitations

- Amplified fragment ≤1kb; if it exceeds 1kb, the amplification efficiency decreases or the amplification fails.

- If PCR products are used for sequencing, it is recommended to purify the PCR products first.

- The PCR product may be slightly mutated. Please be aware that it is used for gene cloning.

- Do not use PCR products obtained from the UNG Anti-PCR Product Contamination System for gene cloning or sequencing.

- The 3' end of the PCR product was randomly A-tailed.

Precautions:(Please read the precautions carefully before using the kit)

- Pay attention to the cleanliness of laboratory equipment and the operation method of the experiment to avoid cross-contamination between samples.

- Please try to use freshly collected animal tissue samples for experiments. If the tissue samples are stored for a long time, please avoid repeated freezing and thawing of the samples.

- If there is precipitation in Buffer AL, it can be placed at 37℃ until the precipitation disappears, and the solution is shaken before use.

- Foregene Protease has a unique formula, please store at 4°C, never at -20°C.

- 2× PCR Easy^TM Mix (UNG) should avoid repeated freezing and thawing, otherwise it will affect the PCR efficiency.

- If the ambient temperature is too high, the 2× PCR Easy^TM Mix (UNG) may become cloudy. It can be placed on ice for 1-2 minutes. After the solution is clear, invert and mix for 3-5 times before use.

Work Flow

Storage and Shelf life:

Part I of this kit should be stored at 2-8℃.

The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃ for longer storage.

Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.

Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.

Part II of this kit should be stored at -20℃ .

Reagent 2×PCR Easy^TM Mix(UNG), if it is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).