Foregene Co., Ltd.
CHINA FOREGENE,WORLD'S FOREGENE!
 |
| Place of Origin: | Sichuan, China |
| Brand Name: | Foregene |
| Certification: | CE, ISO |
| Model Number: | 50T, 100T, 250T |
| Minimum Order Quantity: | 1 kit |
|---|---|
| Price: | Negotiable |
| Packaging Details: | craft paper bag |
| Delivery Time: | 5-8 working days |
| Payment Terms: | L/C, D/A, D/P, T/T |
| Supply Ability: | 100000kits per month |
| Factory: | Foregene | Product Name: | High Recovery Efficiency DNA Gel Isolation Kits For DNA Fragments From Agarose Gel |
|---|---|---|---|
| CAT NO.: | DE-02011, DE-02012, DE-02013 | Type: | DNA-Only Column |
| Application: | Recover DNA Fragments (30bp-10kb) In Agarose Gels | Operation Temperature: | Room Temperature |
| High Light: | DNA Gel Isolation Kits,Agarose Gel DNA Isolation Kits |
||
High recovery efficiency DNA Gel Isolation Kits for DNA fragments from agarose gel
Description
This kit uses the spin column and formula developed by our company, which can efficiently recover high-purity DNA fragments from agarose gel. The kit can recover DNA fragments in a wide range. Under normal conditions, DNA fragments of 30bp-10kb can be recovered. A minimum of 30μl of eluent can be used to increase the concentration of recovered DNA.
The kit is easy to operate and has few steps. It only needs several centrifugation to process multiple samples at the same time, and high-purity recovered DNA fragments can be obtained in 15 minutes.
Kit conponents
| Gel Extraction Kit | |||
| Kit contents | DE-02011 | DE-02012 | DE-02013 |
| 50 T | 100 T | 250 T | |
| Buffer GE* | 50ml | 100ml | 250ml |
| Buffer WB1 | 15ml | 30ml | 75ml |
| Buffer EB | 10ml | 20ml | 50ml |
| DNA-Only Column | 50 sets | 100 sets | 250 sets |
| IFU | 1 piece | 1 piece | 1 piece |
*: Buffer GE contains irritating chaotropic salt. Please wear gloves and take relevant protective measures during operation.
Features&advantages
- Wide range of DNA recovery: DNA fragments as short as 30bp and as large as 10kb can be recovered.
- High recovery efficiency: The highest recovery efficiency can reach more than 80%.
- Small system elution: at least 30μl elution solution can be used for elution, which can effectively increase the concentration of recovered DNA fragments.
- Fast speed: Easy to operate, DNA fragment recovery can be completed within 15 minutes.
- Safety: No organic reagent extraction is required.
- High quality: The recovered DNA fragments are of high purity, which can meet various subsequent experiments.
Application of recovering DNA fragments
The DNA recovered by Gel Extraction Kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, ligation, PCR, sequencing and other experiments.
Quality Control
According to FOREGENE’s Total Quality Management System , each batch of Gel Extraction Kit is strictly tested multiple times to ensure the reliability and stability of the quality of each batch of kits.
DNA recovery efficiency
In view of the characteristics of the purification column, the adsorption capacity and elution efficiency of different fragments of DNA are different, so the efficiency of gel recovery will be different. The general rubber recovery efficiency is shown in the following table:
| GEL Type | DNA fragment recovery efficiency * | OD260/280 | |||
| 30-60bp | 60bp-7kb | 7-10kb | ＞10kb | ||
| TAE | ~50% | 70~80% | 60~70% | ＜50% | 1.7-7.9 |
| TAB | ~40% | 60~70% | 50~60% | ＜40% | 1.7-1.9 |
*After testing, the recovery efficiency of the kit for 23kb DNA fragments is about 20%.
Storage and Shelf life
This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.
Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.
Contact Person: Maggie
Tel: +8615281067355
