High Purification RNA Yield DNA RNA Isolation Kit With RNase Free Linear Acrylamide

High Purification And RNA Yield DNA RNA Isolation Kit With RNase Free Linear Acrylamide DNA and RNA isolation kits

Description:

The Viral DNA and RNA Isolation Kit uses the spin column and formula developed by Foregene, which can efficiently extract high-purity and high-quality viral DNA and RNA from samples such as plasma, serum, cell-free body fluid, and cell culture supernatant. The kit specifically adds Linear Acrylamide, which can easily capture small amounts of DNA and RNA from the samples. DNA/RNA-Only Column can efficiently bind DNA and RNA. The kit can process a large number of samples at the same time.

The entire kit does not contain RNase, so the purified RNA will not be degraded. Buffer RW1 and Buffer RW2 can ensure that the obtained viral nucleic acid free of protein, nuclease or other impurities, which can be used directly for downstream molecular biology experiments.

Specifications:

50 Preps, 100 Preps, 200 Preps

Kit component

* :Buffer DRL and Buffer RW1 contain irritating chaotropic salts. Please wear gloves and take relevant protective measures during operation.

Product information

Features&advantages:

-No need to worry about RNA degradation. The whole kit is RNase-Free

-Simple—all operations are completed at room temperature

-Fast—operation can be completed in 20 minutes

-Safe—no organic reagent used

-Large sample processing capacity—up to 200μl samples can be processed each time.

-High quality—the purified DNA and RNA is highly pure, free of protein and other impurities, and can meet various downstream experimental applications.

Kit application:

Viral DNA and RNA Isolation Kit is suitable for the extraction and purification of viral nucleic acid in samples such as plasma, serum, cell-free body fluid and cell culture supernatant.

Storage and Stability

uThe kit can be stored for 12 months under dry conditions at room temperature (15–25°C); it can be stored at 2–8°C if it needs to be stored for a longer time.

Note: If stored at low temperature, the solution is prone to precipitation. Be sure to place the solution in the kit at room temperature for a period of time before use. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix well before use.

u Linear Acrylamide solution can be stored for 7 days at room temperature; please take out the kit and store it at -20℃ after receipt.

u After adding Linear Acrylamide to Buffer DRL, it can be stored at 2-8°C for up to 48 hours. Please use it for immediate use

Kit Component Information

u Linear Acrylamide: Reduce the background binding of the purification column and capture the trace nucleic acids in the system.

u Buffer DRL: Provides the environment required for sample lysis and the environment for passing the column.

u Buffer RW1: Remove impurities such as proteins in nucleic acids.

u Buffer RW2: Remove residual salt ions from nucleic acids.

u RNase-Free ddH₂O: Elute nucleic acids on the purification column membrane.

u DNA/RNA Column: Highly efficient adsorption of nucleic acids.

Work Flow:

Diagram:

Precautions:

- Repeated freezing and thawing of the sample should be avoided, otherwise the extracted viral nucleic acid will be degraded and the extraction amount will also decrease.

- The sample processing volume should not exceed 200μl, otherwise the yield and purity of viral nucleic acid will be affected.

- After adding Linear Acrylamide to Buffer DRL, it can be stored at 2-8°C for up to 48h. Please use it now.

- Please add absolute ethanol to Buffer RW2 before using the kit. Please refer to the label on the reagent bottle for the added amount.

The addition amount of anhydrous ethanol in the kits of different specifications is shown in the following table:

- The nucleic acid yield and quality are related to the elution volume and sample processing volume. It is recommended to use 200 μl of sample volume per 500 μl of Buffer DRL.

- Elution volume: The elution volume should not be less than 30μl, otherwise it will affect the nucleic acid recovery efficiency.

- All experimental steps are carried out at room temperature (15-25℃) unless otherwise specified.

- Please check whether there is crystal precipitation in Buffer DRL and Buffer RW1 in the kit. If there is crystal precipitation after storage at low temperature, you can place the Buffer at room temperature or 37°C for a period of time, dissolve the crystal and mix well before use.

Storage and Shelf life:

- The Viral DNA and RNA Isolation Kit can be stored for 24 months at room temperature (15-25℃), or 2-8℃ for longer time.

-Linear Acrylamide solution can be stored at room temperature for 7 days. After receiving the kit, please take Linear Acrylamide solution out and store it at -20℃.

-After adding Linear Acrylamide to Buffer DRL, it can be stored at 2-8℃ for up to 48h. Please use the fresh prepared solution.